Create a DNA Fingerprint ( http://www.pbs.org/wgbh/nova/sheppard/analyze.html )
Introduction:
1. DNA is unique for everyone. The only exception is if a person has what?
The only exception to that rule is identical twins. They share the same parents and womb so they have the same DNA.
2. What are DNA fingerprints used for?
DNA fingerprints are used for identifying the person who committed a crime .
Part 1 “It Takes a Lickin”
3. What “crime” was committed?
The "crime" that was committed was of confection. One of Jimmy's seven sisters licked his holographic lollypop.
4. What bodily fluid was removed from the “crime scene” to get DNA?
Saliva was removed from then scene of the crime. It will be used to identify the DNA.
Part 2 “DNA Fingerprinting at the NOVA Lab”
5. What does a restriction enzyme do?
The restriction enzyme cuts the DNA in different locations. The enzyme cuts is different between every person because the code for everyone's DNA is different.
6. What is agarose gel?
The agarose gel is a thick substance that allows small pieces of DNA to get through.
7. What is electrophoresis?
Electrophoresis is the process of moving molecules with an electric current.
8. Smaller fragments of DNA move faster than longer strands?
9. Why do you need to place a nylon membrane over the gel?
The nylon membrane sucks up the agarose gel so it is easier to work with and can be absorbed.
10. Probes attach themselves to the fragments of DNA already on the nylon membrane.
11. Which chemical in your “virtual lab” is radioactive?
The probes are radioactive.
12. Sketch your DNA fingerprint.
13. Based on your DNA fingerprint, who licked the lollipop?
Honey Sweet is the culprit of the crime.
Tuesday, November 23, 2010
Wednesday, November 3, 2010
Mitosis
Chromatin condenses into chromosomes: prophase
Chromosomes align in center of cell: Metaphase
Longest part of the cell cycle:Interphase
Nuclear envelope breaks down: prometaphase
Cell is cleaved into two new daughter cells: Cytokinesis
Daughter chromosomes arrive at the poles:Telophase
2. The colored chromosomes represent chromatids. There are two of each color because one is an exact duplicate of the other.
--How many chromosomes are visible at the beginning of mitosis?: Four
-- How many are in each daughter cell at the end of mitosis?: 4
--The little green T shaped things on the cell are: Centrioles
-- What happens to the centrioles during mitosis?: The centrioles divide during mitosis
3 . Identify the stages of these cells: The first stage in the drawing is Metaphase, the second one is Cytokinesis, and the third one is Prophase.
Prophase- The centrioles move to the opposite sides of the cells. The chromatin condenses into chromosomes and the nucleus dissipates.
Metaphase- The spindle threads attach to the chromosomes.
Telophase- The nucleus reform, the chromosomes distribute, and the spindle threads disintegrate. Cytokinesis takes place.
Interphase Prophase Metaphase Anaphase Telophase Total
Number of cells 20 10 3 2 1 36
Percent of cells 56% 28% 8% 6% 2% 100%
View 1 View 2 View 3 View 4 View 5
Whitefish telophase Prophase Metaphase Anaphase
Onion Prophase Metaphase Interphase Cytokinesis Telephase
Chromosomes align in center of cell: Metaphase
Longest part of the cell cycle:Interphase
Nuclear envelope breaks down: prometaphase
Cell is cleaved into two new daughter cells: Cytokinesis
Daughter chromosomes arrive at the poles:Telophase
2. The colored chromosomes represent chromatids. There are two of each color because one is an exact duplicate of the other.
--How many chromosomes are visible at the beginning of mitosis?: Four
-- How many are in each daughter cell at the end of mitosis?: 4
--The little green T shaped things on the cell are: Centrioles
-- What happens to the centrioles during mitosis?: The centrioles divide during mitosis
3 . Identify the stages of these cells: The first stage in the drawing is Metaphase, the second one is Cytokinesis, and the third one is Prophase.
Prophase- The centrioles move to the opposite sides of the cells. The chromatin condenses into chromosomes and the nucleus dissipates.
Metaphase- The spindle threads attach to the chromosomes.
Telophase- The nucleus reform, the chromosomes distribute, and the spindle threads disintegrate. Cytokinesis takes place.
Interphase Prophase Metaphase Anaphase Telophase Total
Number of cells 20 10 3 2 1 36
Percent of cells 56% 28% 8% 6% 2% 100%
View 1 View 2 View 3 View 4 View 5
Whitefish telophase Prophase Metaphase Anaphase
Onion Prophase Metaphase Interphase Cytokinesis Telephase
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